Description
Aviva Systems Biology HSD3B1 ELISA Kit (Human) (OKEH01670) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human HSD3B1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated HSD3B1 and incubated. The HSD3B1 found in the sample or standards competes with the biotinylated HSD3B1 for limited binding sites on the immobilized anti-human HSD3B1 antibody. Excess unbound biotinylated HSD3B1 and sample or standard HSD3B1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated HSD3B1 captured in the well and inversely proportional to the amount of HSD3B1 which was contained in the sample or standard